#10X CHROMIUM X FULL#

#10X CHROMIUM X FULL#

We can also arrange for a dry run to optimize your cell preparation for the workflow before going into full project with sequencing.Ĭell Prep: The 10X Genomics Cell Preparation Guide describes best practices and methods, but researchers should optimize their preparation protocol for their particular sample type. For cell samples needing sorting, the UMGC offers a MACSQuant Tyto cell sorter as a DIY instrument. See the above Guidelines tab for more info.

WorkflowĬonsult: We request a project planning meeting to review experimental goals and provide guidance on experimental design. We also support library preparation and sequencing of cDNA samples derived from 10X’s Visium spatial solution. The UMGC offers a wide and growing list of 10X single-cell assays to support a broad range of applications, including gene expression, chromatin accessibility, cell surface proteins, immune clonotype, antigen specificity, and CRISPR screens. Additional support services are available on-campus through the University Flow Cytometry Resource (UFCR) and the Minnesota Supercomputing Institute (MSI).Support with experimental design and guidance on assay selection, including adding Feature Barcoding and Cellplex options, or our supported Cell Hashing and CITE-Seq protocols.10X libraries are sequenced on our production-scale NovaSeq 6000 for economical sequencing and project scalability.Latest 10X instrument for analyzing hundreds to hundreds of thousands of cells in a single run.Complete end-to-end workflow: cell suspension loading, 10X library creation, rigorous QC, sequencing, and analysis.The UMGC can handle a range of single-cell projects, from pilot experiments to the highest-throughput studies, and offers the full portfolio of validated 10X single-cell assays, to give researchers flexibility for any experimental strategy. The granularity of the proprietary Next GEM technology allows researchers to interrogate individual cells and unmask rare cell types that may not be seen in bulk RNA sequencing methods. Why is the multiplet rate different for the Next GEM Single Cell 3’ LT v3.The UMN Genomics Center expands single-cell research capabilities with the addition of 10X Genomics Chromium X, a next-generation microfluidic platform for single-cell analysis. Why is the HT multiplet rate lower than our standard assay? Please also refer to the following articles: Cell multiplets can be bioinformatically identified and filtered out when using the 3' CellPlex Kit. For the Single Cell Gene Expression HT assay, it is possible to target up to 60,000 cells per library if using the 3' CellPlex Kit for Cell Multiplexing. Note that for the Single Cell Gene Expression assay, it is possible to target up to 30,000 cells per library if using the 3' CellPlex Kit for Cell Multiplexing. You can control the multiplet rate by adjusting the number of loaded cells and, consequently, the number of recovered cells. Please refer to the Multiplet Rate Table provided in the User Guide for each product, as shown here for the Single Cell 3' Gene Expression v3.1 assay: As more cells are loaded on the 10x chip, it becomes more likely that two or more cells will be partitioned together into the same GEM (cell multiplet). Each chip can process up to sixteen libraries in parallel, allowing users to target up to 320,000 cells per chip.Īn important consideration when deciding how many cells to target is the cell multiplet rate. In each high-throughput 10X Single Cell Gene Expression or Immune Profiling library, it is possible to target up to 20,000 cells without cell multiplexing. Each chip can process eight libraries in parallel, allowing users to target up to 80,000 cells per chip. Question: What is the maximum number of cells that can be profiled?Īnswer: In each standard 10x Single Cell Gene Expression, Immune Profiling, ATAC, or Multiome library, it is possible to target up to 10,000 cells.

10x chromium x